Because the number of candidate parents differed between years, critical Delta-scores were calculated on an annual basis using the simulation option in Cervus.
Calculated on an individual basis.
To focus on nucleosome dynamics, we first calculated a "delta" ChIP-Seq signal by subtracting the normalized ChIP signal for unstimulated cells from the normalized ChIP signal for dexamethasone-treated cells.
We calculated a delta percentage, which expresses the difference between recommended and observed current drug use.
The expression level (RQ value) was calculated based on the delta-delta C T method adjusted to β-actin expression, for each sample.
The obtained RQ values, calculated based on the delta-delta C T method, adjusted to the β-actin expression, and relative to the expression level of calibrator (for which RQ = 1), were compared among NSCLC patients in regard to histopathological NSCLC subtypes, tumor staging (TNM, AJCC), patients' age, gender, and smoking history, as well as to CTLA-4 polymorphic variants.
Antibody titres were calculated based on delta mean fluorescence intensity (ΔMFI) of LN18AQP4 vs LN18CTR.
Sensitivity and specificity calculations based on optimal thresholds are similar to those calculated using a standard delta Ct threshold of 1.0 (compare Table 1 and Table S4).
By using this tensor, we are able to calculate the length of a delta regular curve lying on the surface with the condition of increasing transformation.
Relative quantification was calculated using the standard delta delta methodology [ 15].
