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The distance of the ground truth for each tracking method is calculated in pixel units since Camshift cannot obtain depth information.
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The speed of vehicle is calculated in pixels per second by dividing the distance traveled by the given vehicle over the time taken.
The average velocity of window movement (calculated in pixels per second) was calculated for the entire duration of each trial.
Track length was calculated (in pixels) by tracing the axis of the tracks in Metamorph (Universal Imaging).
The average area of individual colonies were calculated (in pixels) using the ImagePro Plus software as previously described (33).
The distances are calculated in pixels and using the dimensions of the B scan image, the pixels are converted into micrometers.
25 in vivo IV-OCT pullbacks, randomly selected from an already existing database, were both automatically and manually calibrated and the difference between the two procedures (calculated in pixels) quantified as the automatic calibration error.
Each vessel diameter was calculated in pixels as the distance between the half-height points determined separately on each side of the density profile of the vessel image and converted into real values using the calibration factor.
To quantify the areas of degradation, pictures of fluorescent matrix were analyzed by using ImagePro software, and degradation areas associated with positive cells were calculated in pixels, for a minimum of 25 cells per coverslip.
Numerical simulations show that the discrepancy calculated in random pixel images is 4284 on average.
Regional ventilation delay (RVD) was analyzed not in [ 4], but in [ 5], where it was calculated in each pixel.
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