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ΔΔG changes associated with mutating every amino acid of all four subunits to all 20 possible amino acids were calculated in duplicate for each of the three conformers.
Serum osmolality was calculated in duplicate and the coefficient of variation was <1%.
The %HFF was calculated in duplicate form from the mean pixel signal intensity data using the following equation: ([Sin − Sout]/[2 × Sin]) × 100.
The DNA concentration of each extract was calculated, in duplicate, by measuring the fluorescence of known dilutions with Hoechst 33258 dye (1 μg ml−1) in TNE buffer (10 m M Tris base, 1 m M EDTA, 0.2 m M NaCl, pH 7.4) read on a Biolite fluorescent microtitre plate reader.
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Correlation coefficients calculated in dCHIP showed significant agreement between duplicate samples for all three genotypes analyzed.
The coefficient of variation (CV) between duplicate
Intra-assay CV for Hp was calculated in the same manner, based on seven duplicates.
Mean score for duplicate cores from each individual was calculated in tumor epithelial cells.
Hence, increase of acetic acid concentration in bioreactor was calculated and validated in duplicate non-inoculated D-stat test experiments producing an average standard deviation of 1.24 mM between calculated and measured acetic acid concentrations.
22 Mean systolic blood pressure was calculated from duplicate measurements in the right brachial artery using a cuff that was appropriate to the size of the arm.
The intra-assay CVs (n = 20) for three samples (mean NGAL, 75 516 ng/mL) were 4.3 6.2%; the interassay CVs calculated from duplicate results in 10 subsequent assays were 4.5 6.7%.
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