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Dvorak et al (1991) used a similar conventional Gaussian approach, deriving their reference values from means calculated in a previous study of healthy volunteers [ 21].
Under the experimental conditions used, the IC50 value for doxorubicin in OVCAR-3 cells was 5 μ M, which confirmed the 2.5 μ M value calculated in a previous experiment (Houba et al, 2001a).
For adjustment of the deeper branches, we furthermore applied secondary age constraints, with divergence time estimates between mantellid genera based on the confidence intervals calculated in a previous study [ 69] (Additional file 1, Table S4).
Under similar experimental conditions (pH 8.0 at 37 °C), the specific activity for T5FEN, calculated in a previous study (610 units/μg) [ 29] was similar to that reported in the present study (420±10 units/μg), The specific activity of SaFEN (90±5 units/μg) was approx. 5-fold lower than that of T5FEN, equivalent to a turnover rate of 106 min−106
This is within 10% of the 16.9 amp value calculated in a previous step.
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By comparing drug effects of the same drug applied in different hypothalamic nuclei we could calculate in a previous study that when applied by microdialysis the effective spread of BIC was ∼0.5 mm [23].
The experiments are, therefore, calculated in a larger domain than the previous regional studies and that comprises the whole mantle depth: They are either in 2D Cartesian or in a 3D full spherical shell geometry.
Equation (42) is calculated in a similar way as in the previous section: begin{array}rcl@ u_{k}^{Ku}&=&KDelta R int_{Sigma}frac{partial G_{kp}}{partial xi_{p}}dSigma =-frac{KDelta R}{8pimu}D^{x}_{kp}frac{partial}{partial x_{p}}int_{Sigma} LdSigma &=&-frac{lambdaDelta R}{8pimu} 1-Gamma) fR}{8pimu} 1-Gammaial x_{k}}frac{partial^{2}{partiall x_{k}}frac{partial^{2}_{Sigma}{partial.
Residual error calculation: To evaluate the quality of the warped block calculated in the previous step, a MAD metric is adopted.
The intervention calculated in the previous section represents a gold-standard for this particular scenario because the vaccination strategy was calculated using the same parameter values and the same SIR model formulation as the simulated disease process.
The times between aggregates were: the half-life period (30 min) calculated in previous experiment, a reduced half-life period (23 min) and then study the system after 24 h between determinations.
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