Suggestions(1)
Exact(3)
TERT expression levels were calculated from threshold cycle values and normalization to 18S and B2M values respectively (2(−ΔCt)).
Relative amounts of mRNA were calculated from threshold points (Ct values) located in the log-linear range of real time PCR amplification plots using the 2-ΔCt method.
Relative copy number was calculated from threshold cycle value (DCt), and the mtDNA copy number/cell was calculated as 2 × 2−DCt to account for the two copies of RNaseP in each nucleus (55).
Similar(57)
The threshold diameter calculated from measured threshold pressure showed that it does not follow the traditional similarity theory.
The ΔCt of the control and experimental samples was calculated from the threshold cycle of the target gene minus the threshold cycle of the reference gene (ACT1).
The ΔCt of the control and experimental samples was calculated from the threshold cycle of the target gene minus the threshold cycle of the reference gene (ACT1) in triplicate experiments.
The Ct (threshold cycle) value of each sample was calculated from the threshold cycles with the instrument's software, and the relative expression of MIC-1 mRNA was normalized to the GAPDH value.
The Ct (threshold cycle) value of each sample was calculated from the threshold cycles with the instrument's software (StepOne Software v2.1; Applied Biosystems, Foster City, CA, USA), and the relative expression of MIC1 mRNA was normalized to the GAPDH value.
Relative expression was calculated from cycle threshold values (ΔΔCt method) using gapA gene, encoding d-glyceraldehyde-3-phosphate dehydrogenase A, as an independent internal control.
Quantification of the samples was calculated from the threshold cycle (Ct) by interpolation from the standard curve.
Cell numbers of bacteria in fecal samples were calculated from the threshold cycle values (Ct) and expressed as quantity of bacteria per gram feces [30].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com