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The standard error of the mean was calculated from replicate data-set for each experimental condition.
Intra- and inter-assay coefficients of variation, calculated from replicate determinations of quality controls were 7.94 and 6.52% (high) and 13.31 and 11.26% (low) respectively.
The mean Ct of the gene of interest was calculated from replicate measurements and normalized with the mean Ct of a control gene, GAPDH, for which expression was relatively constant.
The mean was calculated from replicate wells.
The average values of gene expression were calculated from replicate probes.
Coefficient of variation (CV) was calculated from replicate values within the replicate plates and mean values between replicate plates (Kinnear and Gray, 2007).
Similar(54)
Mean peak FRET ratios calculated from replicates were exported and plotted against agonist concentrations using Graphpad Prism 4 (Carlsbad, CA).
Average values were calculated from replicates.
Oocyst infection values for gene silencing experiments were calculated from replicates of ≥30 dissected mosquitoes.
The analytical variability (coefficient of variation calculated from replicates of the QC samples) was 2.2% and 1.0% for Alu methylation and LINE-1 methylation, respectively.
After normalization of the hybridization signals across all array chips, an average signal intensity for each probe was calculated from replicates of each parasite.
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