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If we were to see the body plan and morphological patterning as readouts of genetic programs for development, then the ancestor indexes calculated for developmental genes would refine our understanding of the conservation of the stages between 8 and 18.
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EDI scores were calculated for each developmental domain i.e. Physical Health and Well-being; Social Competence; Emotional Maturity; Language and Cognitive Development; and Communication Skills and General Knowledge.
EDI scores were calculated for each developmental domain, that is, Physical Health and Well-being; Social Competence; Emotional Maturity; Language and Cognitive Development; and Communication Skills and General Knowledge.
Using these thresholds, the proportions of high CVISI (bursting cells), medium CVISI (irregular cells), and low CVISI (regular cells) were calculated for each developmental stage: all neurons were found to be bursters at P2 3, most neurons were irregular between P5 and P11, while pacemaker neurons became predominant after P12 and were the only type of neurons encountered after P21.
In the third evaluation approach, the coefficient of variation of normalized relative expression levels was calculated for candidate genes throughout each developmental series tested.
The reads per kilobase of transcript per million reads mapped (RPKM) value was then calculated for each transcript in each developmental stage using R (Additional file 5).
The mean expression, SD and CV (=SD/mean) was then calculated for each of the 7,922 probesets across the developmental set, stress set and entire dataset (which included the hormone experiments).
The expression levels were calculated for rice different tissues or same tissues at different developmental stages by summing all expressed tags in the sense strand.
A continuous score is calculated for each age-specific ASQ with scores summarized for each developmental domain.
Gross motor age equivalent scores were not included in the overall developmental score as this measure is only calculated for children 0 33 months of age and, therefore, was not collected for each child.
The index of developmental synchronisation in the gall of P. populi was calculated for all the destructive samples because the full range of instars was never in the gall at the same time.
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