Exact(1)
We performed a single reaction deletion on the network and calculated flux values by FBA using the Cobra Toolbox [ 13] to assess essential reactions under aerobic glucose minimal media conditions (as described in the supplementary material of Feist et al. 2007).
Similar(7)
The MILP was re-solved after adding a constraint (z∗) for a single flux of the original flux distribution which was set to either 1.01-fold (z 1 ∗ ) or 0.99-fold (z 2 ∗ ) of its original calculated flux value (v0).
Thus, simulation approaches based on linear/quadratic programming (LP/QP) optimization methods are used to calculate flux values.
However, the value of these fragments only had minor impact on the calculated flux distribution (simulation results not shown).
Thus, although values from in vitro enzyme activity measurement and flux analysis are not directly convertible, the measured enzyme activities support the calculated flux distribution towards glutamine and the low level of glutamine usage.
Once metabolic clusters were formed, previously calculated metabolic flux values (step A to C of FMB) corresponding to each clustered reaction were used in the subsequent BN analysis.
For the September study period, the average errors were estimated to be 12% of the calculated DOC flux value, 16% of the calculated FTHg value, and 13% of the calculated FMeHg value.
Calculated fluxes were then fitted according to all these enzyme activity values.
Related(1)
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