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The calculated expression values and original probe intensity files have been deposited in NCBI'S GEO microarray database (series accession #GSE6992).
Finally the predictor uses calculated expression values to build a pseudo-expression matrix.
The DESeq implementation of the negative binomial test was than used to find significant differences in the calculated expression values.
For this, the normalized and fold change calculated expression values for the Vitis and X. fastidiosa interactions experiments were mapped on to these pathways.
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A calibrator sample was then used as a control, whereby each calculated expression value was normalized to the calibrator sample.
Array-based data sets including GSE17054 (Majeti et al., 2009), GSE19599 (Andersson et al., 2010), and GSE28490 (Allantaz et al., 2012), were downloaded to calculate expression values based on a method described previously (Wang et al., 2015a, b).
Only unique reads with a maximum of three mismatches were used to calculate expression values.
A GC-RMA algorithm was used to calculate expression values (12).
Using gcrma rather than the more common MAS5 to calculate expression values gives a more powerful AP4 test.
We used imputation to calculate expression values for unknown genes using a small set of pre-defined genes (Methods).
To more accurately calculate the expression level of the assembled genes, only both pairs of paired-end reads with unique location were retained to calculate expression values.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com