Sentence examples for calculated expression of the from inspiring English sources

Prior to building the classifier we calculated expression of the selected genes relative to the median expression the reference genes as measured on the same microarray (rps19, cgi- 119, ctbp1 and b2m were used as the reference genes) [ 8, 9].

L is the base counts of the coding region of gene A. FPKM method can eliminate the influence of gene length and difference of sequencing on the calculation of gene expression, and the calculated expression of the gene can be directly used to compare gene expression between different samples.

Thus ΔΔCT = ΔCT treated sample) - ΔCT untreated control) The mean of these ΔΔCT measurements was then used to calculate expression of the test gene (2-ΔΔCT) relative to the reference gene and normalized to the untreated control as follows: Relative Expression = 2-ΔΔCT This calculation assumes that all PCR reactions are working at 100% efficiency.

To calculate expression of the unannotated portion of extended 3' UTR piRNA precursors in somatic tissues, we first downloaded RNA-Seq data for spleen, lung, liver, kidney, heart, cortex, cerebellum and bone marrow from the ENCODE project (Ren LICR-m group, [ 50], DCC_Accession wgEncodeEM001706, wgEncodeEM001709-1715).

RNA-seq mapping was performed with RSEM [ 41] to calculate expression levels of the assembled transcripts for the three tissue types.

Our method avoids the necessity of calculating the expression of the Green's function.

The mean Ct values from duplicate measurements were used to calculate the expression of the target gene using the formula: fold change in gene expression, 2−ΔΔCt = 2−{ΔCt (SFN-treated samples)- ΔCt (untreated control)}, where ΔCt = Ct (hTERT)- Ct (GAPDH).

2−ΔΔ C t method allowed to calculate relative expression of the genes.

FPKM method (Fragments Per kb per Million reads) was applied to calculate the expression of the Unigenes [ 81].

The Vandesompele method of quantification was used to calculate the expression of the target genes relative to the internal control genes.

To calculate the expression of the unannotated portion of the extended 3' UTR cluster precursors, we used mouse testis RNA-Seq data [ 18] downloaded from the NCBI Sequence Read Archive (ww.ncbi.nlm.nih.gov/sra, accession number SRR036361).