Sentence examples for calculated expression of from inspiring English sources

Prior to building the classifier we calculated expression of the selected genes relative to the median expression the reference genes as measured on the same microarray (rps19, cgi- 119, ctbp1 and b2m were used as the reference genes) [ 8, 9].

L is the base counts of the coding region of gene A. FPKM method can eliminate the influence of gene length and difference of sequencing on the calculation of gene expression, and the calculated expression of the gene can be directly used to compare gene expression between different samples.

However, since the expression of MMP9 by (S FB is discussed controversially in the literature (see [ 57] and [ 58] vs. [ 59]), the calculated expression of MMP9 by fibroblasts – at least at a limited number of time points – supports the majority of studies, reporting detectable MMP9 mRNA amounts in (S FB.

Thus ΔΔCT = ΔCT treated sample) - ΔCT untreated control) The mean of these ΔΔCT measurements was then used to calculate expression of the test gene (2-ΔΔCT) relative to the reference gene and normalized to the untreated control as follows: Relative Expression = 2-ΔΔCT This calculation assumes that all PCR reactions are working at 100% efficiency.

We calculated expression levels of all known genes for all 8 samples using the RPKM (Reads Per Kilobase per Million of reads) measure of gene expression [ 59].

RNA-seq mapping was performed with RSEM [ 41] to calculate expression levels of the assembled transcripts for the three tissue types.

Based on the slopes for hordein and reference primers we selected the optimal cDNA concentrations to calculate expression level of hordein transcripts.

To more accurately calculate the expression level of the assembled genes, only both pairs of paired-end reads with unique location were retained to calculate expression values.

Utilizing SK-01 cells as control cells not expressing ERs, we calculated gene expression of cells ectopically or endogenously expressing ER α or ER β.

Correlation was calculated between expression of miRNAs vs gene probes differentially expressed in at least 1 on 3 time points.

Ratio of GFP /GFP larva or pupa were calculated, if expression of hAtx1-82Q at glial cells is not toxic the ratio should be 1.