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The calculated expression values and original probe intensity files have been deposited in NCBI'S GEO microarray database (series accession #GSE6992).
In order to correlate changes in expression level of each transcript with changes in the clinical values, we calculated, expression levels and clinical values as (value post – value pre)/value pre, and the mean for all subjects was expressed as percent change.
Finally the predictor uses calculated expression values to build a pseudo-expression matrix.
First, we calculated expression divergence between human and rat homologous tissues.
A calibrator sample was then used as a control, whereby each calculated expression value was normalized to the calibrator sample.
The DESeq implementation of the negative binomial test was than used to find significant differences in the calculated expression values.
Similar(37)
He had a handsome face, with a cold, calculating expression.
We note that formula (74) provides us a practical method to calculate expression (72).
Afterwards, quantile scaling and WPP algorithm were used to normalize and calculate expression estimates [29].
Normalized Ct values (calculated automatically within the Applied Biosystems software RQ manager 1.2). were used to calculate expression ratios between the cocaine and control groups.
Following RMA normalization, human data were further processed to calculate expression scores adjusted for gender and batch effects as described by Gudjonsson et al. (51).
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