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The transduction efficiency was calculated counting the number of green cells in 10 randomly selected fields, 24 hours after transduction.
The participation rate was calculated counting the number of patients present at the outpatient clinic and the respective inpatient units at the time of assessment.
Ki67 index was calculated counting a minimum of 10 randomly selected high-power (× 400) fields containing representative sections of tumour and calculated as the percentage of positively stained cells to total cells (Burcombe et al, 2006).
The composition of the accessory regulons in terms of un-annotated targets was calculated counting the number of CDS with no COG classification (Additional file 7) resulting in an average percentage of 49%, while for the core regulons the percentages of un-annotated targets is lower (21%).
PTEN expression, detected with the primary antibody anti-PTEN (clone 28H6, NeoMarkers, Fremont, CA 94539TS 106) was quantified by using a visual grading system based on the intensity of staining and the percentage of positive nuclei calculated counting of 1000 cells in 3 different optical fields at 40× magnification.
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This rate was re-calculated counting only transition records confirmed to be sent by CR as the denominator.
Time-integrated, post-flash, delayed neutron counts were measured and compared to calculated count predictions in order to benchmark the calculational methodology and computer models.
Calculated count rates will be reasonably accurate without additional compensation for CTI when using these ARFs.
For each recommendation, we calculated counts and proportions to describe recommendation uptake.
Technical information and plots of the effective areas used to calculate count rates in the PIMMS software.
SummarizeOverlaps was used to calculate counts of reads for each gene with the default mode of "Union".
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