Exact(1)
We calculated cell migration speed on ECMs of varying ligand density for different values of ECMs stiffness (Fig. 5B).
Similar(59)
To calculate cell migration velocity, cells were tracked using the timelapse macro in Axiovision software.
Total cell migration was obtained by calculating cell number in the lower chamber after 6 hr of incubation at 37°C in 5% CO2.
The combined multiscale model calculates the integrated cell migration response, a result of coupled processes involving polarization, protrusion, contraction, translocation, and retraction.
Cell motility was determined by percent wound closure (calculated by dividing distance moved of invading front at the indicated time points) and mean speed of cell migration (calculated by dividing the total distance of migration by indicated time points, at least 20 cells per cell line were analyzed).
Custom software was then used to calculate the cell-free area, and the cell migration rate was calculated using the changes in this area over time.
Cell migration was calculated as percentage of migrating cells, regarding the number of cells in the absence of EPO as 100%.
Cell migration was calculated as the number of EC migrating into the wound area at various time points indicated.
Inhibition rate of cell migration was calculated according to the equation, in which Migctrl is from the control cells that migrate into the lower surface and Migt is from the treated cells that migrate into the lower surface.
The experiment was performed six times, and percent cell migration was calculated using following equation: B16F10 cell adhesion after treatment with plain ETO and micellar formulation to substrate-coated plates was carried out as reported earlier, with slight modifications (Dua et al. 2007; Lopez-Barcons et al. 2004).
Differences in cell migration were calculated by measuring repopulation of the scratch wound after 12 hr when compared to the original wound edge (time zero).
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