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The number of pericytes per EC (pericytes coverage) was calculated by manually counting the number of Cd31+ and Ng2+ cells in a field of view, and then counting how many were in contact with Cd31+ cells.
We used 31,740 and 16,729 data for the absolute arrival times of the P- and S-waves, respectively, and 68,934 and 31,638 data for the differential times calculated by manually picking data for the P- and S-waves for event pairs within 10 km, respectively (Waldhauser and Ellsworth 2000).
Spine density was calculated by manually counting the number of spines expressed over a 30 µm segment of dendrite.
Bayes factors were calculated by manually summing the tree likelihood and coalescent/skyline columns in the BEAST log file.
We defined the position of the four points by a ratio calculated by manually specifying five points that define the shape of the tongue.
Scar and oedema volumes will be calculated by manually drawing endocardial and epicardial contours followed by semiautomated selection of normal remote myocardium per slice.
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Vehicle velocities were calculated by measuring manually the distance and using the time span between the acquisition times which can be derived with high accuracy from the GPS/IMU data.
EMG-locked averages (ERP waveforms) were calculated by first manually marking EMG onset.
The number of monomers within each arc was then calculated by dividing the manually traced arc length by the average size of a monomer in the prepore and pore states, 23.6 Å and 25.7 Å, respectively, as estimated from rotationally averaged negative-stain EM images.
Δ2θ m is manually calculated by comparing 2θ-angle values of Bragg-peaks in the raw data and certification by NIST.
It is manually calculated by the planimeter.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com