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To analyze the relation between the DNA methylation and the expression of genes, we plotted the methylation ratio calculated by MIAMI and the expression level calculated by expression microarray analysis for all genes examined (Fig. 7A).
The percent of scattering from the nucleus was then calculated by expression 3. The area of images from which lysosomes are the major source of scattering were estimated by taking the area of only lysosomal fluorescence and adding 1/2 of the area that had both lysosomal and mitochondrial fluorescence but no nuclear fluorescence.
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Normalized expression was calculated by formula:{(EfficiencyhANT4) CT hANT4/ (EffiCTencyALG9} CT ALG9} using qGene software [31].
Expression level was calculated by averaging expression signals in the 61 tissues, whereas tissue-specificity, which ranges from 0 to 1 (higher values indicate stronger tissue-specificity), was calculated according to Liao et al. (2006).
The calculated results were compared with the test results and the shear strength results calculated by empirical expression in the ACI 318-14 code.
When the temperature is between these limits, the valve position is calculated by the expression ( (5.0/3.0) * (temperature - low_bound) ).
Thus, the first integrals (conserved forms) for nonlinear fin equation (3.1) can be calculated by using expression (2.12) and by considering each group parameter c i.
According to the model proposed by Ruppin and Englman [27], the frequency of SO phonon mode in a cylinder NW can be calculated by the expression (1) (2).
The value of output local average luminance of the pixels in Ω xy can be calculated by the expression g avg ( x, y ) = ∑ ( i, j ) ∈ Ω x y w i, j y T ( x + i, y + j ), (A1).
Best housekeeping genes (POLR2A, RPS11, GUSB, RPL37A, ACTB) for data normalization were chosen according to their stability score calculated by the Expression Suite 1.0.1 software (Life Technologies, Carlsbad, California, USA).
Relative expression levels were calculated by dividing RAE1 expression levels by UBQ expression levels.
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