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Enzymatic activity was calculated by determination of liberated fatty acid through titration with 0.05 N KOH and using phenolphthalein as end-point indicator.
Based on this condensed tree, a root is calculated by determination of the central node: Starting at each leaf node, a depth first search is performed and the nodes are labeled according to their depth in order to find the longest path in the tree.
Protein concentrations of fibrils were calculated by determination of the amount of the single tryptophan residue under denaturing conditions as published [19], [22].
The parasite density was calculated by determination of the number of parasites per 200 white blood cells (WBC) for thick blood film or 500 red blood cells (RBC) for thin blood films.
The dose response for each strain was calculated by determination of the area under the growth curve at each dose (see Methods section for details).
The intraocular distances (l) are calculated by determination of the travel time (t) of an ultrasonic wave with velocity (c) according to the formula l= ct.
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Estimation of chemical oxygen demand (COD) was done by closed reflux titrimetry method, while biochemical oxygen demand (BOD) was calculated by oxygen determination by Winkler titration for the preserved leachate sample.
Maternal iodine intake may be calculated by the determination of ioduria (level of iodine in urine) taking into account that the factor of dilution in urine is greater in pregnant women than in the remaining population.
Concentrations of stock solutions in DMSO were calculated by colorimetric determination of the 2,4-dinitrophenyl group (ɛ365=17 300 M−1 cm−1) and then adjusted to 1 mM and stored in the dark at −20°C.
Branch thickness was calculated by direct thickness determination, as described previously (Hildebrand and Rüegsegger, 1997).
The explanatory power of ACG was calculated by coefficients of determination.
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