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The percent-injected activity per gram (%IA/g) was calculated by comparison to a weighed, diluted standard.
One to three readings were taken at each time point and a percentage survival calculated by comparison to t = 0.
Relative abundance of the target transcripts was calculated by comparison to a standard curve, and normalized to the expression level of ribosomal protein L5 (RPL5).
Absolute cytokine amounts were calculated by comparison to a standard curve generated using a 3rd order polynomial regression curve-fitting algorithm.
The control culture supernatant served as the 100% hemolysis control, and relative percentage hemolysis values were calculated by comparison to this value.
Test agent specific clearance was then calculated by comparison to saline controls, normalised to 100%, and expressed as % change in clearance compared to control.
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Concentrations were calculated by comparison of absorbance to a cellobiose standard curve.
The percentage of inhibition was calculated by comparison of sample rates to a blank.
Concentration of lysozyme in each sample was calculated by comparison of absorbance values to those of the standard curve.
The percent injected activity per gram (%IA g− 1) for each tissue was calculated by comparison of the tissue counts to a standard sample prepared from the injectate.
The remaining activity was obtained in the same way and inhibition was calculated by comparison between control activity (considered to be 100%) and remaining activities of the treatments.
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