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Finally, the estimates of forest attributes were calculated as sample means for each sample.
Reagent blanks were run with each set of analyzed samples, and the percent inhibition of NADPH oxidation was calculated as sample rate/blank rate × 100.
Optical density was measured at 490 nm by spectrophotometric analysis and viability was calculated as sample optical density divided by control optical density.
To normalize the results of different runs, the cut off index for each sample was calculated as sample A450/COV.
Percentage of cytotoxicity was calculated as (sample optical density [OD]490 – OD0%)/(OD100% – OD0%)×100, where OD0% represents the OD490 of noninfected cells and OD100% represents the OD490 of cells treated with lysis buffer as indicated by the manufacturer.
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The percentage of samples was calculated as follow: positive cases ÷ detected cases × 100%.
The relative gene expression of the genes was calculated as ΔCt sample = (Ct sample GENE) − (Ct sample HKG).
A point estimate, the standard error and the 95% credible interval for a parameter are, for example, calculated as the sample mean, the sample standard deviation and the sample 2.5% and 97.5% quantiles of the posterior samples for that parameter.
The freezing rate was calculated as freezing sampling number/total sampling number X 100%.
The required sample size for the analysis of the change in pain was calculated as the sample size needed in a simple linear regression model of the change in pain at 12 months on the baseline exposure.
Third, the sample size was small and did not exceed the desired 76-paired observations, calculated as the sample size to detect differences in accuracy between participants with MCI and NC for the screening tools.
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