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Sensitivity and specificity were calculated and quantified using receiver operating characteristic (ROC) curve analysis.
Sensitivity and specificity were calculated and quantified using receiver operating characteristic (ROC) analyses.
The numbers of migrated cells in three individual areas were calculated and quantified using Image J software (NIH).
The plate was incubated for 15 min at 37 °C and the fluorescence 485/520 was measured at time point 0. Eighty microlitres of 62 mM 2,2′-azobis 2-amidino-propane) dihydrochloride were added to each well and the fluorescence was measured every minute for 80 min. The difference in absorbance at time point 0 and after 80 min was calculated and quantified using a standard curve.
Similar(56)
Images were obtained using infrared scanning (Odyssey, USA) and quantified using GelPro32, and calculated by the ratio of phosphorylation to the total protein level.
Relative expression levels of each gene were calculated and quantified by the 2−ΔΔCt method using ACTB as endogenous control [ 28].
The ratio of the remaining wound area relative to the initial wound area was calculated and the wound area was quantified using Image-Pro Plus v. 6.0 software.
Contrast-to-noise ratio was calculated for all tumours, and fluorochrome accumulation was quantified using fluorescence-mediated tomography.
Composite T-scores are calculated for multiple goals and change over time is quantified using change scores and using conventional procedures recommended in literature [ 19].
The absorbance of the reaction mixture at 0 min and 30 min was assayed to calculate the released phosphate, which was quantified using a KH2PO4 standard curve.
Volumes of VAT and SAT were quantified using MASS.
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