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The number of nodes and edges for each coexpression network along with the average and standard deviation values for the following network parameters – node degree (k), clustering coefficient (C), path length (l), eccentricity (e) and betweeness (b) – were calculate using the program tYNA [ 24].
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The B index was calculated using the program Skew Calculator 2003 (http://www.eeb.ucla.edu/Faculty/Nonacs/shareware.htm).htm
The cell doubling time was calculated using the program from the Doubling Time Online Calculator http://www.doubling-time.com/compute.php.php
Water channel extending from the active site to the surface of the protein as calculated using the program CAVER49.
Electrostatic surface potential was calculated using the program APBS in Chimera with the nonlinear Poisson Boltzmann equation and contoured at ± 5 kT e−1.
For microsatellite DNA data, allelic richness for each locus and population and FIS were calculated using the program FSTAT v. 2.9.3 (Goudet, 2001).
The curves were calculated using the program CURVES [30].
Activities and saturation indexes of the solutions were calculated using the program PHREEQC [60] and the database Phreeqc.
Nucleotide distances were calculated using the program DNAsp [41] and the program "Distmat" included in EMBOSS package [40].
Amplification efficiencies and threshold cycle (Cq) values were calculated using the program LinRegPCR (Ruijter et al. 2009).
Biomass of biofilms was calculated using the program COMSTAT [51].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com