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To calculate the viability of the cultures the colony count of all samples was normalized by the colony count of the control (only viable cells plated).
To calculate the viability of the brain slices (as a percentage) after the 5 h incubation-equilibration period, the following steps are taken for each experimental setup: Background absorbance control - provides information about the background absorbance of the assay medium (aECF).
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The developed colour was measured at 570 nm spectrophotometrically to calculate the relative viability of fibroblast cells (Cos-7) on the scaffolds which in turn illustrates the biocompatibility of the material [ 12].
Relative viability was calculated as the viability of cells grown in the presence of 2-AP divided by the viability of cells grown in the absence of 2-AP.
Viability was calculated as percentage of the viability of untreated cells (100% viability).
The proposed yeast cell counting algorithm can calculate the total number and measure the viability of yeast with exceptional accuracy and precision within less than 2 seconds in a conventional PC and 6 seconds in an embedded system when combined with FDA/PI and yeast dilution buffer.
The viability of cells was calculated according to the manufacturer's instructions of MTS CellTiter 96 (Promega, Madison, WI, USA).
The viability of each culture was calculated as a percentage respect to an untreated control.
The viability of target cells was calculated as a percentage of the non-treated target control.
Colonies were counted and the viability of the test mixture calculated.
The viability of treated cells were calculated similarly from the absorbance values and expressed as percentage of control viability.
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