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Moreover, we calculate the expression for the second curvature coefficient, extending previous results using this formulation.
Reads that could be uniquely mapped to a gene were used to calculate the expression level.
end{aligned} In order to assure (langle q^, qrangle =1), we need to calculate the expression of D.
The expressions of the reference gene(s) were determined by qPCR and data were analyzed with geNorm to calculate the expression stability (M) of each gene.
The expressions of the reference genes were determined by qPCR and data were analyzed with geNorm to calculate the expression stability (M) of each gene.
Proof of Lemma 3. We first calculate the expression in the left hand side (assuming ), which is the upper bound in (30): (30).
The two values of signal intensities resulting from the color swap were averaged, and the signal intensities were used to calculate the expression ratio (the averaged signal intensity of the Fe-deficient group divided by that of the control group).
Resulting values were used to calculate the expression ratios between female and male antennae (FA/MA).
Three programs were applied to calculate the expression stability of candidate reference genes: geNorm [14], NormFinder [15] and BestKeeper [16].
The mean Ct values from duplicate measurements were used to calculate the expression of the target gene using the formula: fold change in gene expression, 2−ΔΔCt = 2−{ΔCt (SFN-treated samples)- ΔCt (untreated control)}, where ΔCt = Ct (hTERT)- Ct (GAPDH).
Cufflinks (version 2.0.2) [ 16] was used to calculate the expression of transcripts.
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