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In order to determine the differentially expressed transcripts within the dataset, we used Cuffdiff, a separate program included in Cufflinks, to calculate expression in two or more samples and test the statistical significance of each observed change in expression between them.
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Tag start files were used to calculate expression as detailed in RNA-MATE manual.
Proof of Lemma 3. We first calculate the expression in the left hand side (assuming ), which is the upper bound in (30): (30).
A plot of the standardized samples was used to calculate cytokine expression in the BAL samples.
This average efficiency was used to calculate the expression in each reaction using the formula Expresion = ECT.
Listed for each of the 8,786 E. tenella genes are the normalised counts calculated by DESeq in each of the three developmental stages (gametocytes, merozoites and sporozoites), along with the calculated differential expression in gametocytes over either merozoites or sporozoites.
The merged assembly transcripts provide a uniform basis for calculating transcript expression in each condition and the transcript class code.
In order to normalise the amount of cDNA in each sample and to guarantee the comparability of the calculated mRNA expression in all analysed samples, the housekeeping gene aldolase was amplified.
To normalize the amount of cDNA in each sample and to guarantee the comparability of the calculated mRNA expression in all samples analyzed, the housekeeping gene GAPDH was also analyzed.
We calculated gene expression in two ways; using a single 3' probe to mimic commonly used 3' based microarray profiling and using the mean of all probes measurements associated with the gene.
Because in calculating expression (5), there is no need to determine tail elements a 11 2 j + 1 − a 11 k j + 1 to process the (j + 1 th frame.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com