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We note that formula (74) provides us a practical method to calculate expression (72).
Array-based data sets including GSE17054 (Majeti et al., 2009), GSE19599 (Andersson et al., 2010), and GSE28490 (Allantaz et al., 2012), were downloaded to calculate expression values based on a method described previously (Wang et al., 2015a, b).
Afterwards, quantile scaling and WPP algorithm were used to normalize and calculate expression estimates [29].
Following RMA normalization, human data were further processed to calculate expression scores adjusted for gender and batch effects as described by Gudjonsson et al. (51).
Normalized Ct values (calculated automatically within the Applied Biosystems software RQ manager 1.2). were used to calculate expression ratios between the cocaine and control groups.
Normalized signal intensities were used to calculate expression ratios.
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He had a handsome face, with a cold, calculating expression.
Because in calculating expression (5), there is no need to determine tail elements a 11 2 j + 1 − a 11 k j + 1 to process the (j + 1 th frame.
The calculated expression values and original probe intensity files have been deposited in NCBI'S GEO microarray database (series accession #GSE6992).
Re-calculate expression estimates using only the included probes.
In the future, we plan to re-calculate expression levels based on S. japonicum gene microarray analyses.
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