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MTT experiment was used to calculate cell survival rate.
For this study, the number of objects within five randomly selected biofilm internal subspaces with dimension of 30 × 30 × 30 μm3 were averaged to calculate cell density.
Transient multiphysics simulations in ANSYS CFX 15.0 were carried out to calculate cell temperatures and to determine the short circuit current and temperature coefficient in a scaling law.
Deoxyribonucleic acid content of the LG was used to calculate cell density and total cell number.
To calculate cell migration velocity, cells were tracked using the timelapse macro in Axiovision software.
To calculate cell number, cells were stained with 4', 6 diamidino-2-phenylindole (DAPI).
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Supplementary material Fig. S4 illustrates the procedure to calculate cell-division orientation of A cell, which generates two daughter cells, the A′ cell and A″ cell (supplementary material Fig. S4A).
Nearly a 1 1 relationship was observed between predicted and calculated cell concentrations using qRT-PCR.
Calculated cell resistance from the polarization curve was 0.0836 Ω which is comparable with SS316L under similar conditions.
The maximum relative discrepancy between the measured and calculated cell voltage by the numerical model was 3%.
These estimates are highly likely affected by a non-negligible error, which in turn affects the calculated cell addressing [H, I] used for packet forwarding.
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