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For L. loa, Fletcher's team then created software that, by analyzing a 5-second video recorded through the microscope, could detect that wiggling motion and calculate a concentration of parasites in the blood.
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The 13C-exchange indices of the metabolites were calculated from a concentration of unlabeled and uniformly-labeled metabolites.
We therefore calculated that a concentration of 2 mg/ml would be the maximum safe dose that normal urothelial cells could withstand.
For each sample, we then calculated a standardized concentration of lead in the meat (log-transformed value minus the fitted geometric mean for the species, divided by the standard deviation from the fitted model).
Using a protein A calibration curve, we calculated a local concentration of 84 ± 9 mg/mL for rProtein A Sepharose, more than twice the amount on MabSelect (31 ± 9 mg/mL).
Based on these assumptions, we calculated an effective concentration of 49.3µg of vitamin A per g of oil at the consumption level.
Assuming that the peptides are α-helical and oriented, we calculate a peptide concentration in the monolayer of around 100 m m; significantly higher than the solution-phase peptide concentration (65 μ m).
EGCG, the most abundant and potent active compound in GTP [ 17], was used to calculate a series of mole concentrations to present the total GTP, but the overall results of this study were from total GTP.
We did not calculate a sum of the absolute concentrations since such a summation assumes equal immunomodulatory potency of all the studied compounds.
Thus, to calculate the concentration of a particular sample, the mean of the concentration estimates obtained from Ct values via the calibration curve should be calculated, including the negative observations as zero concentration.
The limit of detection (LOD) of this method was calculated at a concentration level of 300 pM.
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