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Results from two cage population experiments with a small number of mated foundresses suggest the existence of costs associated with the production of diploid V. canescens males.
From this point onwards each cage population was self-sustaining.
The glasshouse cage population suppression experiment was designed to simulate a field scenario in which biotic and abiotic mortality factors maintain a stable pest population, with adult moths mating and laying eggs in glasshouse cages, and larval rearing being conducted in a separate temperature-controlled room [ 18, 20, 27].
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After 5 months (approx. 8 generations), all cage populations went to extinction because of all male-offspring production (I. Amat and C. Bernstein, unpublished data).
Cage populations were initiated by placing 200 unsexed wild-type pupae into each cage.
Interaction of crop and cage populations occurs only in an exchange phase marked Exc in the figure.
Defining extinction as 2 weeks of zero egg production [ 17], both treatment cage populations were extinct by week 12 PR.
Larvae were screened only after the returning population was separated, to remove the possibility of bias in selecting individual flies for reintroduction to the cage populations.
We describe a method of measuring the fitness costs associated with transgenes by analyzing their evolutionary trajectories when placed in competition with wild-type alleles in replicated cage populations.
After another couple of generations, in four out of five cages, the population died out entirely.
We are ultimately interested in differences between the B and O populations, as well as the variation in demography that can be attributed to the replicate cages and to the replicate populations within the same selection treatment, e.g. B or O. Below we describe how we estimated the demographic parameters for each cage-sex population.
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