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Prior to experiments, all birds had experienced the same daily cleaning and feeding routine that we continued for the duration of the experiments: morning weighing, feeding, and water changing; afternoon water changing; weekly cage changes; and additional twice weekly cage bottom cleaning.
Two weeks, enough time for at least two cage changes, is a bare minimum for this purpose.
This result indicates that inclusion of H2 atom within empty small cage changes size of host cages depending on type of guest molecule.
Seizures were studied in Synapsin I/II double knock-out (SynDKO) mice, a genetically engineered mouse line that shows seizures upon daily handling procedures such as tail lifting during cage changes, much in resemblance to the more studied El mouse.
Animal monitoring and cage changes were performed weekly by the experimenter or a professional animal caretaker.
In between the two social interaction tests the mice were undisturbed except for routine cage changes.
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Open bar represents control (no cage change).
Results showed that cage change with vehicle administration increased plasma corticosterone levels (from 162.2 ± 11.16 to 269.25 ± 12.51 ng/ml; p = 0.000) compared to control (no cage change).
Here onwards, all the results explained were obtained by using cage change protocol.
The changes in stress level were studied following octacosanol or vehicle administration and cage change in mice.
(C) Hourly plots of NREM sleep in mice after p.o. administration of vehicle (gray circles), octacosanol (red circles) with cage change, and control (vehicle with no cage change; open circles).
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CEO of Professional Science Editing for Scientists @ prosciediting.com