MDC inhibits clathrin-mediated receptor internalization by stabilizing clathrin cage assembly, preventing clathrin polymerization [57], [58].
Residues that mediate the COPII cage assembly were identified: HDX data identified four unique contact regions that interface two proteins Sec13 and Sec31, each of which is involved in the assembly and flexibility of the COPII cage.
It was shown that cage assembly leads to a large conformational change at the interface between Sec13 and Sec31 and that Sec13 plays an important part in rigidifying the hinge by providing structural integrity.
Electrochemical measurements on two-cage assemblies, where two C-phenyl ortho-carboranyl groups are linked by a para-phenylene or a para-tetrafluorophenylene bridge, revealed two well separated and reversible two-electron reduction waves indicating formation of stable dianions and tetraanions.
16, 17 Given that a closer look at the unusual 2 n+3 SE anions can improve our understanding of the luminescence properties of diaryl- ortho-carboranes, we decided to examine the reduction properties of the two-cage assemblies, 2, 3 and 4, where two C-phenyl ortho-carboranyl groups are linked by a meta-phenylene, a para-phenylene and a para-tetrafluorophenylene bridge, respectively (Scheme 1).
The laser cage mount assembly is shown in the annotated photograph of Fig. 1b.
(A ) Left panel, an expanded negative-stain image of TBC-DEG Q73L showing the cage-like assemblies.
In contrast with the more open BchH structure, ChlH forms a more enclosed cage-like assembly, connected to an N-terminal 'head' region adjacent to a 5 nm-diameter opening in the structure.
10.7554/eLife.08811.011 Figure 4. TBC-DEG complexes are compact cage-like chaperone assemblies with hollow cores.
Many systems have been developed over the years for encapsulating hydrophobic small-molecule guests in molecular cages and amphiphilic assemblies.
