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Smart-pool siRNA were transiently transfected into two individual CAF lines (CAF#1 and CAF#2).
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People still crowd the News Caf 1/8, where Versace ate his last meal and read his last newspaper.
Importantly, there was a similar shift of both SEPT2 and SEPT7 into the insoluble fraction in CAF#1 4.
There were no significant effects of CAF on metabolism (VO2 or RER) except for a lower RER in CAF-1 versus CAF-5 at 60% VO2peak.
Cheloufi, S. et al. The histone chaperone CAF-1 safeguards somatic cell identity.
Mechanistically, CAF-1 suppression led to a more accessible chromatin structure at enhancer elements early during reprogramming.
Notably, suppression of CAF-1 also enhanced the direct conversion of B cells into macrophages and fibroblasts into neurons.
Schönrock N., Exner V., Probst A., Gruissem W. & Hennig L. Functional genomic analysis of CAF-1 mutants in Arabidopsis thaliana.
Hatanaka, Y. et al. Histone chaperone CAF-1 mediates repressive histone modifications to protect preimplantation mouse embryos from endogenous retrotransposons.
Impairing replicative H3.1 incorporation via CAF-1 enables an alternative H3.3 deposition at replication sites via HIRA.
Both CAF-1 and CAF-3 DBP were also significantly (P < 0.05) higher versus PL at preexercise.
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