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All laboratory analyses were performed under pressurized containment cabinets, class II B2.
325 million cells per milliliter were transferred to TAP agar plates exposed to ultraviolet (UV) light at a distance of 30 cm (253.7 nm, 100 μW/cm, 60 Hz, NuAire safety cabinets Class II Type A2 NU-425-400, http://www.nuaire.com) for 2 min under sterile conditions modified from Luck et al. [ 53].
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Like an errant schoolboy caught passing smutty messages to the girls at the next desks, Mr Lesin was ordered to stand up before the rest of the cabinet class while the head teacher and prime minister, Mikhail Kasyanov, issued his rebuke.
All DNA extractions were conducted in biological safety cabinet class II.
The MODS culture was conducted in a biosafety cabinet class I which was placed in a separated room from the sample processing room, smear preparation room and MGIT culture room.
All manipulations must be carried out in a Biological Safety Cabinet Class II.
300 sputum samples were processed in a Biosafety cabinet class II following standard procedures [ 27].
All preparation was done in biosafety cabinet Class II Type A2 using PPE for BSL-3.
However, due to space constraints, DNA extraction was performed on a bench in the main laboratory with template added to the PCR reaction mixture in a Top-safe 1.2 biological safety cabinet class II (BSCII) (Bio-Cabinets Australia Pty, Australia).
Using a biosafety cabinet class II B-2, we prepared suspensions of viscera (liver, spleen, and kidney) obtained from the monkey and then injected suckling mice by the intracerebral route.
Laboratory Procedures: A. Sputum decontamination Sputum specimens were decontaminated using MycoPrep™, a solution containing sodium hydroxide and N-acetyl-L-cysteine, in Falcon tubes, following sputum decontamination guidelines [ 11 ], in Biosafety cabinet class II type A2 using Personal Protective Equipment PPEE) for Biosafety Laboratory Level-3 (BSL-3).
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