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For the TCS genes in Chinese cabbage, expression profiling is an initial step; further elucidating the potential functions of these genes involved in abiotic stress adaptations would be an interesting and meaningful study.
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To understand which GRF genes may be involved in regulating specific tissue or organ growth in Chinese cabbage, the expression patterns of the BrGRFs in various tissues were investigated by real-time quantitative PCR (RT-qPCR).
A comprehensive analysis of tissue or organ-specific expressions of TCS genes in Chinese cabbage, and their expression profiles to abiotic stresses (drought and high salinity) and exogenous phytohormones (tZ and ABA) were conducted.
To analyze the Chinese cabbage R2R3-MYB expression patterns, we used Illumina RNA-seq data reported previously [ 39].
Nevertheless, we suggests that the results of this study indicating down-regulation of CYP19 mRNA and protein expression by cabbage juices observed in breast nontumorigenic MCF10A cell line may explain the chemopreventive activity of white cabbage and sauerkraut observed in epidemiological studies.
Chinese cabbage unigenes and tissue expression level data were downloaded from NCBI (http://ncbi.nih.gov/repository/UniGene/Brassica_rapa/).
Proteomic analyses in autopolyploid cabbage have shown very few expression changes [ 34].
In summary, we identified 226 AP2/ERF TFs in the cabbage genome and characterized their expression patterns in response to different abiotic stresses.
Thus, we also examined the effects of exogenous ABA on TCS gene expressions in Chinese cabbage.
Comparisons of the tissue-specific expression profiles between Chinese cabbage and Arabidopsis TCS genes might help in determining the unknown functions of the TCS genes in Chinese cabbage.
In G. candidum, cgl expression is linked to cabbage and sulfur aroma development in smear cheeses through methanethiol (MTL) production [ 71- 73].
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