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Experimental solutions were saturated with halothane in N2 gas during Ca2+ uptake by SR, Ca2+ release by SR, or during both SR Ca2+ uptake and release.
Remodeling of mitochondrial Ca2+ uptake and mitochondrial shapes in proliferative and quiescent GSLCs.
Ca2+ uptake was visualised with Fluo-8 and measured with the BZ9000 system.
Our results are in accordance with the requirement of enhanced mitochondrial Ca2+ uptake and the formation of donut-shaped mitochondria.
Halothane (0.5-1.7 0.5-1.7ted in dose-dependent depression of SR Ca2+ uptake in both newborn and adult skinned fibers.
The rate of mitochondrial Ca2+ uptake is sensitive to extracellular [Ca2+], indicating that mitochondria sense Ca2+ gradients near CRAC channels.
Growing evidence suggest that, in the presence of toxic stimulus, the mitochondrial Ca2+ uptake may induce apoptosis in a variety of pathological conditions38.
This remodelling of mitochondrial morphology from tubular to donut may be an additional mechanism explaining the increased mitochondrial Ca2+ uptake capacity observed after SOC stimulation in quiescent GSLCs.
This suggests that quiescence is associated with changes in Ca2+ homeostasis through regulation of Ca2+ influx and of mitochondrial Ca2+ uptake.
As a result, stimulated contractions were observed (Supplementary Movie S1) in differentiated myotubes together with twitch-synchronised Ca2+ uptake using the fluorescent dye Fluo-8 (data not shown).
Ca2+ uptake by the mitochondrial store is sensitive (threshold is <400 nM cytosolic Ca2+), rapid (detectable within 8 s), and does not readily saturate.
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