Sentence examples for by whole transcriptome amplification from inspiring English sources

We generated cDNAs by whole transcriptome amplification (WTA) of these individual cells (see Experimental Procedures for details).

RNA extracts were amplified by WTA (Whole Transcriptome Amplification) and hybridised on the resequencing microarray (see Methods section).

Extracted viral RNAs were reverse transcribed into cDNA using SuperScript III reverse transcriptase (Invitrogen Inc., Carlsbad, CA, USA) then amplified by the whole transcriptome amplification (WTA) approach in the presence of random hexamer primers.

cDNA was synthesized by using the TransPlex® Complete Whole Transcriptome Amplification Kit according to the manufacturer's protocol (SIGMA).

cDNA was generated with 20 ng of RNA by use of the Qiagen Whole Transcriptome Amplification Kit (Qiagen), as described in the manufacturer's protocol.

Here, we have tested the efficacy of NuGEN's Ovation RNA-Seq V2 system for whole transcriptome amplification by comparing matched amplified and unamplified samples to identify if there exists any subtle changes in gene expression after WTA among biological replicates.

Evaluating whole transcriptome amplification for gene profiling experiments using RNA-Seq.

Fig. 3 Schematic of the whole transcriptome amplification methods for single cell sequencing.

To mitigate this bottleneck, whole transcriptome amplification (WTA) technologies have been developed to generate sufficient sequencing targets from minute amounts of RNA.

The central component of Pico Profiling is Whole Transcriptome Amplification (WTA), which generates expression profiles that are highly comparable to those produced by others, at different times, by standard protocols or by Real-time PCR.

Reverse transcription on total mRNA lysates was performed using the QuantiTect Whole Transcriptome Amplification kit (Qiagen ltd., UK) following the manufacturer's directions.