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We, therefore, propose a systematic approach for diagnosing MRCD that starts with a biochemical enzyme assay and is followed by whole mtDNA sequencing.
We would, therefore, propose a systematic approach for diagnosing MRCD that starts with a biochemical enzyme assay and is followed by whole mtDNA sequencing.
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6 In this study, we performed whole mtDNA sequencing for 90 children diagnosed with MRCD by RC enzyme assay with the aim of identifying causative mtDNA mutations.
Whole mtDNA sequencing of seven samples was obtained using an Ion PGM™ sequencer (Life Technologies Corporation, Carlsbad, CA).
In this study, we performed whole mtDNA sequencing for 90 children with MRCD, and identified 29 mtDNA variants.
Our results demonstrate that it is important to perform whole mtDNA sequencing rather than only typing reported mutations.
Therefore, there were no analyses of the relationships between species within cartilaginous fish orders using whole mtDNA sequences, although intrarelationships were estimated by using partial mtDNA and nuclear DNA sequences [ 6, 7, 10, 15– 20].
Whole mtDNA sequences of the seven sharks were determined by PCR-based shotgun sequencing.
Based on recent whole mtDNA sequences in modern samples, the five founding haplogroups have been expanded to at least 15 founding lineages (Perego et al. 2010).
The phylogenetic tree of whole mtDNA sequences was constructed in several stages.
Whole mtDNA sequences identified 29 mtDNA variants in 29 patients (13 were previously reported, the other 13 variants and three deletions were novel).
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