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We also demonstrate by western analysis that expression of AKAP79, AKAP149 and RII are upregulated with DC differentiation and maturation.
Moreover, we demonstrated by western analysis that expression levels of subunits NDUFA9 and NDUFB8, known to integrate later in the complex I assembly pathway than NDUFS2 (Lazarou et al., 2007; Vogel et al., 2007), were decreased relative to controls in these cells.
To begin to define I. scapularis salivary proteins that elicit tick-immunity, we immunized guinea pigs with salivary gland extracts from 66 72 h fed nymphs and confirmed by western analysis that the immunized guinea pig sera reacted with protein extracts from fed tick salivary glands (Fig 4A-inset).
Here we further show by western analysis that Skp2 downregulation is accompanied by an increase in p21, p-cdc2 and Mty1 levels, and that p-Wee1 and CyclinB1 are decreased, but levels of p27 are nearly unchanged.
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Quantitation of the GFP directly by Western analysis demonstrates that OAGR increases the efficiency of split vector gene reconstruction greater than 60-fold (Fig. 2C).
Thus, by western analysis, it appears that these monoclonal antibodies only recognize the post-translational cleaved product and not the short or long version of SAX-7.
As previously observed by western analysis, we found that DNA-PK deficiency (KA4, M059J) but not ATM deficiency (AT5BI) inhibits γ-H2AX positive cell formation in 32Hc treated cells (Fig. 3A).
This is supported by western analysis, which revealed that the overall level of H2BE in the MOE doubles from 7 weeks to 45 weeks (approximately 10 months) of age.
The ratio of editosome proteins in the WT-myc-containing editosomes detected by western analysis was similar to that in those from KREPA3-RKO (E) cells.
Reverse co-immunoprecipitations of ERα (Fig. 5G) and ERβ (Fig. 5H) followed by western analysis of Gα13 confirmed that Gα13 and ERα (but not ERβ) co-interact in a ligand-dependent fashion in T47-D cells.
The expression profile detected by Western analysis seems to contradict that of the Northern blots (Table 1).
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