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eS26 protein levels were assessed by Western analyses using α-eS26 antibodies.
The proteins in the gradient were detected by Western analyses using the indicated antibodies.
The Calmodulin eluates were visualized by Silver staining and by Western analyses using the indicated antibodies.
eS26 protein levels were assessed by Western analyses using α-GFP antibodies.
The gradient was fractionated, TCA precipitated and the protein content was assessed by Western analyses using the indicated antibodies.
Proteins were visualized by Coomassie Blue staining or by Western analyses using antibodies against Tsr2 and eS26.
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Co-enrichment of eS26 with pre-ribosomal particles was assessed by (1) Western analyses using antibodies that recognize eS26 and (2) selected reaction monitoring mass spectrometry (SRM-MS).
We then confirmed that the large difference in IRES dependent-eGFP expression detected by FACS and Western analyses using the pMigR constructs remains true with the pPRIG vector.
Bound proteins were eluted in SDS sample buffer, separated by SDS-PAGE and visualized by Coomassie Blue staining and Western analyses using α-eS26 antibody.
Calmodulin-eluates were analyzed by Silver staining and Western analyses using the indicated antibodies.
Bound proteins were eluted in SDS sample buffer, separated by SDS-PAGE and visualized by Coomassie Blue staining and Western analyses using α-eS26 antibodies.
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