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After decapitating by vortexing, equal numbers of frozen heads from each genotype were homogenized and solubilized in ice-cold RIPA buffer for 30 min. Homogenates were spun at 500× g and then 14,000× g for 15 min at 4°C to remove debris [35].
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The pellet obtained was dissolved in 0.1 M NaHCO3 and extracted by vortexing with an equal volume of chloroform and methanol (2 1, v/v).
For analysis of VP1 expression, harvested yeast cells (20 50 mg) were suspended in 20 50 μL of DB150 buffer (150 mM NaCl, 1 mM CaCl2, 0.25 M L-Arginine, 0.001 % Triton X-100, 10 mM Tris/HCl, pH 7.2), lysed by vortexing with an equal volume of glass beads for 7 min, and then cooled on ice for 1 min between each vortexing step.
The place, populated today by roughly equal numbers of blacks and whites, is dripping with history.
Ms. Quinn is backed by equal numbers of men and women.
The Nazis were met by an equal number of anti-fascists who'd turned up to counter their demonstration.
Editors have to ask themselves, "Am I editing works by equal number of men and women?
These jobs aren't replaced in anything resembling equal numbers by the larger firms.
Therefore, an equal number of men and women were chosen, and equal numbers by race and ethnicity.
After centrifugation, the cells were mechanically disrupted by vortexing for 30 seconds in the presence of an equal volume of 425 600 μm acid-washed glass beads (Sigma Aldrich, Canada).
To extract UQ, whole ovarian homogenates were mixed with an equal volume of hexane/ethanol for 10 min by vortexing.
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