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24 h after elicitor treatment, cellular necrosis was assessed by visually scoring the tissues for browning.
We monitored the effects of various mutations and small deficiencies on TPE in flies heterozygous for the 39C-5 insert by visually scoring the amount of pigment.
Viral replication was assessed by visually scoring the cytopathic effect without staining.
Comets were observed at 40x magnification, and migration was determined by visually scoring the tail length according to published protocols [ 22].
The occurrence of switching from the BR-F and BR-RF to the BR-S colony phenotype was determined by visually scoring the colonies of different ages growing either on GMA or on YEGA media.
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Transgenic lines were selected by visually scoring anthocyanin production.
The resulting images were visually analyzed for in vitro fungal growth, followed by visually scoring of live and dead worms based on nematode shape, as live worms appear sinusoidal and dead worms are rod shaped.
I next assessed expression of cyclinG1, p53 target gene, after mRNA injections by visually scoring expression strength as "high" or "low" based on staining of uninjected embryos (Fig. 6B), where embryos with "high" cyclinG1 expression were inferred to be shh−/− mutant and those with "low" expression were wild-type (control stainings of wild-type embryos, not shown).
Firstly, leaflet disease was estimated by visually scoring disease symptoms in infected plants.
Disease severity was estimated by visually scoring disease symptoms in infected leaflets as a percentage of leaflets showing disease lesions.
Two researchers visually scored the recordings according to standard criteria.
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