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The quality and quantity of RNA were assessed by A260/A280 and by visualization on a 1.2% formaldehyde-agarose gel.
The RNA concentration was quantified by determination of the absorbance at 260 and 280 nm, and RNA integrity was checked by visualization on a 1.5% agarose gel.
RNA quantity and quality was independently assessed by visualization on a 1.5% agarose (wt/vol) formaldehyde gels and on an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA).
The quality and specificity of amplified products was confirmed by visualization on a 2% agarose gel.
RNA quality was assessed for all samples by visualization on a denaturing formaldehyde RNA gel (protocol recommended by Qiagen, Valencia, CA, USA) and ethidium bromide staining.
Integrity of RNA was confirmed by visualization on a 1% TAE (40 mM Tris pH 8.2, 40 mM acetate, 1 mM EDTA) agarose gel.
Similar(49)
Once completed, we added 0.5 μL of BSR-DI (2000 U/mL, New England Biolabs, Ipswitch, MA) to each reaction and incubated for one hour at 65°C, followed by visualization on an agarose gel.
The samples were checked by visualization on an agarose gel.
The RNA was quantified spectrophotometrically and its integrity validated by the OD260/OD280 absorption ratio (> 1.8) and by visualization on an agarose gel.
The RNA was quantified spectrophotometrically and its integrity verified by the OD260/OD280 absorption ratio (> 1.8) and by visualization on an agarose gel.
The clusters, as critical data characteristics for modeling multiple system conditions, are first estimated by "visualization" on the dissimilarity spectrum from spectral analysis and then evaluated in terms of their fitness and separation with each others.
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