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Staining with the secondary antibody and Hoechst was performed as described before, followed by visualisation under a fluorescence microscope.
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RBC binding to transferrin fibres was studied by incubating fresh human RBCs with a 4-day-old transferrin solution, followed by visualisation under an optical microscope.
The PCR products were loaded on 1% (w/v) agarose gel for electrophoresis and then stained with ethidium bromide, followed by visualisation under ultraviolet light.
Following extraction, RNA was assessed for quality by visualisation on a 1.2% agarose gel, and quantified using a Nanodrop spectrophotometer (Thermo scientific).
RNA quality was assessed by visualisation on a denaturing formaldehyde RNA gel (protocol recommended by Qiagen) using ethidium bromide staining.
The purified RNA was quantified by spectrophotometric measurements and its purity and integrity verified by the OD260/OD280 ratio (> 1.8) and by visualisation on a denaturing gel.
They were further confirmed by visualisation in the BeadStudio.
Celebs swear by visualisation.
Finally, social, task and cognitive elements which can be significantly supported by shared visualisation and a guideline for future researchers seeking to design shared visualisation-based systems are presented.
In all, 10 μl of the RT-PCR reactions were electrophoresed on a 2% agarose gel and stained with ethidium bromide for visualisation under UV light.
After a final BPS wash (3 ×) coverslips were mounted onto slides using citifluor and stored at 4°C in the dark, till visualisation under the microscope.
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