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Functionality of exposed cardiomyocytes was analyzed by visual quantitation of the rate and extent of contractility.
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1– 5 Moreover, visual quantitation is relatively time-intensive, has some degree of imprecision and requires a certain level of experience.
Quantitation was performed by visual comparison of signal intensity relative to that of a titration series as described next.
These results are related to the reduced amount of starch granules in leaves of the HC-Pro expressing plants, and this was confirmed by visual observation of starch pellets during thylakoid preparation, and by direct quantitation of starch from the leaf samples (Additional file 7).
This signal saturation effect is overcome by LC-IM-DIA-MS2-High3 quantitation, demonstrated by the quantitation of abundant proteins in line with LC-DIA-MS quantitation.
Percentage of proliferating hepatocytes was estimated by the quantitation of hepatocytes that incorporated BrdU antigen.
Furthermore, these results were backed up by the quantitation of real-time RT-PCR results.
These findings were confirmed by quantitation of internal β1-integrin.
The oxidative modification of LDL was evaluated by quantitation of conjugated dienes [50], [51].
In each case, the DNA isolation efficiency was controlled by quantitation of β-globin DNA.
Atherosclerosis in the aortas was measured by quantitation of Sudan IV positive lesions as described [44].
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