Sentence examples for by vigorous shaking and from inspiring English sources
Fecal samples were homogenized by vigorous shaking and ≈1 ml of the ethanol/fecal slurry was aliquoted into DNA-free 2 ml tubes and centrifuged at 12 000g for 1 minute.
Four centrifugation tubes filled with 20 ml of cold glycerol saline (3 −20°C20°C) were then mixed with 10 ml of culture by vigorous shaking and immediately placed into the centrifuge.
Five ml of the culture were extracted with 5 mL dichlormethane by vigorous shaking and the liquid phases were separated by centrifugation at 5000×g for 10 min. Two mL of the organic phase containing HAQs and pyocyanin as major components were dried by evaporation.
After protease treatment, epithelial cells were released by vigorous shaking and were harvested by centrifugation.
200 μl of chloroform were added, followed by vigorous shaking and incubation for 2 3 minutes at room temperature.
Cells were detached biweekly by vigorous shaking, and fresh cultures were started at a concentration of 5 × 10/ml.
The cells were harvested and resuspended in a fractionating buffer (10 m Tris HCl, pH 7.6; 150 m NaCl; 1.5 m MgCl2, 0.5% Nonidet P-40 (Sigma-Aldrich), and protease inhibitor cocktail), followed by vigorous shaking for 5 min and centrifuged at 12 000 r.p.m. for 30 s.
The glandular stomach was cut into 5 mm pieces and incubated a total of three times with 2 mM EDTA in Hanks' balanced salt solution (HBSS) (Invitrogen) supplemented with 2% FCS for 15 min at 37°C, followed by vigorous shaking for 15 s and filtration to remove epithelial cells.
Extraction was carried out for 30 min at room temperature by vigorous shaking of the mixture and was followed by ultracentrifugation at 100 000 g for 30 min.
To estimate solubility, 8 10 mg of compound was dissolved in 1 g of different solvents by vigorous shaking for 1 h and visual control of solubilisation.
Denaturation can be brought about in various ways e.g., by heating, by treatment with alkali, acid, urea, or detergents, and by vigorous shaking.
