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It is excluded by viable cells, but acridine orange (AO) can penetrate intact membrane to stain viable cells [18].
Propidium iodide was excluded by viable cells, but stained the nuclei of necrotic cells.
It is excluded by viable cells but can penetrate cell membranes of dying or dead cells.
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The dye is called an exclusion diazo dye, which is taken up by dead cells only, but excluded, by viable cells [16].
FDA, a membrane permeable dye, is metabolized by viable cells to a green fluorescent dye.
The assay is based on the reduction of the indicator dye, resazurin, to the highly fluorescent resorufin by viable cells.
The assay is based on reduction of the indicator dye, resazurin, to the highly fluorescent resorufin by viable cells.
Corresponding absorbance reading ofdissolved formazan was taken after its conversion by viable cells which was plotted with respect to control (untreated cells) against concentration gradient.
The formazan granules formed by viable cells were dissolved in DMSO and the absorbance at 570 nm was estimated by measuring with an ELISA reader (BioTek, ELx800).
The formazan grains formed by viable cells were solubilized with DMSO, and the color intensity was measured at 550 nm with an ELISA reader (BioTek Instruments, Winooski, VT).
Upon completion of the protocol, the hearts were stained with 1% 2,3,5-triphenyltetrazolium chloride (TTC), a dye taken up only by viable cells.
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