For Sc Scc3 9, crystals were grown by vapor diffusion, equilibrating drops of 100 nl reservoir plus 100 nl protein solution (30 mg/ml) against reservoir containing 100 mM cacodylate/EtOOH pH 6.5, 7.5% (w/v) PEG 20,000, 15% (w/v) PEG 550 MME, 400 mM KSCN and 2.5% (w/v) jeffamine M-600 (adjusted to pH 7).
Single crystals of [Zn4 2](BF4)8 suitable for analysis by X-ray crystallography were obtained by slow vapor diffusion of diethyl ether into a saturated acetonitrile solution of the Solomon link.
Recrystallization of 2 by vapor diffusion at ambient temperature yielded crystals of α- 2, while at 30 or 35 °C these experiments yielded either α- 2 or, more commonly, a combination of both α- 2 and β- 2 phases.
In the presence of high concentrations of inert gases, heat transfer is governed by vapor diffusion and condensate drainage is of major importance.
Crystals of the ternary complexes of hRARα/mRXRα LBDs and TIF-2 peptide were obtained at 17°C by vapor diffusion in hanging drops by mixing of 0.5 µl of the protein solution and 0.5 µl of reservoir solution which contains 200 mM potassium thiocyanate and 20% PEG3350.
Crystals were obtained by vapor diffusion in hanging drops consisting of 2 µL concentrated protein solution (approx 8 10 mg/mL in 50 mM Tris-HCl [pH 7.8], 0.2 M NaCl) and 2 µL of reservoir solution.
Crystals were grown by vapor diffusion at 22°C by mixing 1 μl of sample with 1 μl of reservoir solution (20 mM MgCl2, 20 mM Tris HCl, pH 8.0, 1.6 M ammonium sulfate) in a 48-well hanging drop tray (Hampton Research, Aliso Viejo, CA, United States).
Crystals of hu3S193 Fab were produced in 2 µl sitting drops in the presence of Ley by vapor diffusion in a 96-well, round bottom, sitting-drop plate (Corning, Acton, MA, USA) using the Crystal Screen HT kit (Hampton Research, Aliso Viejo, CA, USA).
