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Considering the importance of foxtail millet, functional significance of TEs and the necessity of TE-based markers in genotyping applications, this study was performed to identify the different classes of TEs and develop molecular markers by utilizing the sequence information of TEs.
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Gene identification and functional assignment have been accelerated by utilizing the genome sequences.
Molecular methods such as SSCP or RFLP were able to discriminate these fragments by utilizing the differences in sequence.
Yu [20] filled the gaps in the result of Zhang and Song [18] for the Halpern-type iterative algorithm (1.2) by utilizing the result on the sequence of real numbers in [21], which is of fundamental importance for the techniques of analysis.
Frequently, to evolve new morphological or behavioural forms evolution has had to reuse and adapt existing elements by utilizing the inherited genomic sequences in a new context-dependent manner.
We next identified individual pilS cassettes within the fkbp-lpxC regions of each isolate by utilizing the Align Two Sequences tool available at NCBI to interrogate the extracted fkbp-lpxC region using the pilS1 gene from N. meningitidis strain M01-240149 as a query sequence in tBLASTn searches performed under default conditions.
Tissue typing by molecular method, utilizing the sequence-specific oligonucleotide (SSO) and sequence-specific primer (SSP) technologies has been in routine use in many tissue-typing laboratories worldwide for more than 20 years since the development of the polymerase chain reaction.
Finally, as an extension of the trust model, by utilizing the trust assessment data sequence, we propose an improved SCGM 1,1 -Markov chain prediction method baSCGM 1,1 -Markovm chain gray model and Markov stochastic chain theory to forecast entity׳s trust level for future decision making.
By utilizing the strengths of both sequencing technologies, this approach yielded a considerable increase (~20%) in transcriptome annotation when compared to 454 alone.
Mutations of the TBC1D1 constructs were confirmed for accuracy by utilizing the high-throughput DNA sequencing service at Brigham and Women's Hospital (Boston, MA, U.S.A .. Plasmid DNA was amplified in Escherichia coli TOP10 cells (Invitrogen), extracted using an endotoxin-free Plasmid Mega Kit (Qiagen) and suspended in saline at 4 μg/μl.
The obtained elemental images then are encrypted by utilizing the CA model and chaotic sequence.
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