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The in vitro osteogenesis of the BMSCs or the MC3T3-E1 cells grown on the SPAN based IDEs under the electrical stimulation was investigated by utilizing an alkaline phosphatase (ALP) activity assay.
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The kinetics for the electro-oxidation of ammonia in alkaline media was revisited by utilizing a rotating disk electrode (RDE) system.
One of these technologies utilizes an alkaline silicate solution forming a chemical barrier when mixed with CO2-enriched water.
After the induction, alkaline phosphatase staining was performed on the cells utilizing a Leukocyte Alkaline Phosphatase Kit (Sigma-Aldrich).
Preparation of plasmid DNA was performed utilizing a modified alkaline lysis protocol followed by purification through anion exchange column (Qiagen, Valencia, CA).
By producing alkaline γ-aminobutyrate and utilizing an intracellular proton, functions that involve the gadBC operon, cells can adapt to low pH [ 43].
Pseudomonas aeruginosa was used to produce alkaline protease by utilizing available agricultural residues (wheat bran) via solid state fermentation (SSF).
The bands were visualized by use of an alkaline phosphatase color development kit (Bio-Rad).
In the present study, alkaline degradation was minimized by utilizing degassed water and the addition of 20 mmol/L NaBH4 prior to extraction.
By utilizing resources, including.
By utilizing the free energy released from the energetically downhill movement of H+ in response to the electrochemical H+ gradient (Δμ̃H+, interior negative and/or alkaline), LacY catalyzes the uphill (active) transport of galactosides against a concentration gradient.
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