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cDNA was synthesized from 5 µg of total RNA by utilizing a reverse transcription kit (RevertAid™, Fermentas Life Science, International INC, Canada) in a final volume of 20 µl.
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A separate analysis utilizing a reverse database yielded near identical protein identifications at a 1% false positive cut off (data not shown).
In addition, we enriched for full-length cDNA by utilizing a template-switching reverse transcriptase (SMART™ technology – BD Clontech, Mountain View, CA).
"Armed with a consumer's name and ZIP code, Michaels is capable of obtaining its customers' complete mailing address by utilizing a 'reverse phone book' that matches names and ZIP code, which it does in order to increase profits through direct marketing, or it could sell its customers' mailing addresses to third parties," the plaintiff argued in a legal brief filed with the state court.
However, by utilizing an integrative approach, this problem is minimized.
These criteria were selected based on a method that utilizes a reverse database false positive model [37].
TILLING is a non-transgenic technology that utilizes a reverse genetics approach for the production and detection of mutation [ 132].
By utilizing this reverse genetic approach in the future, more mutagenized populations can be characterized in a time efficient manner.
In a word, varied mesophases of OMCs have been achieved directly utilizing a homemade reverse copolymer as a soft template and low molecular resin as a precursor by an EISA method as the hydration increases.
RT-PCR and qPCR were used to detect miRNA expression in liver metastases by utilizing TaqMan® MicroRNA Reverse Transcription Kit and TaqMan® miRNA Assays.
This paper employs mathematical modelling of seawater and brackish water desalination systems utilizing a single-stage reverse osmosis process.
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