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By utilizing a nested PCR amplification protocol employing primers encompassing the tag sequence, we recovered 45 different phage-Abs associated with the selected tags from the T populations.
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The assay utilizes a nested PCR approach with the first PCR amplifying a well-conserved segment from the Babesia 18S ribosomal RNA gene followed by a nested PCR with Babesia species-specific primers and annealing temperatures enabling amplification of the 18S ribosomal RNA gene fragment specific to either B. bigemina or B. bovis.
This will be done through the second study hypothesis that utilizes a nested case-control design within the total cohort.
Our method, implemented in the new software package anytag, utilizes a nested set of paired-end libraries with decreasing insert sizes.
The data collected with 48 semi structured interviews, 96 journals, and 24 observations with 8 English language teachers were analyzed qualitatively by utilizing nested ecosystems model as an analytical framework, which led to the identification of internal and external factors underpinning teachers' personal styles at the micro system.
Cells achieve specific performance objectives by utilizing nested feedback loops, feedforward control, and ultrasensitive signaling motifs, etc.
The design utilized was a nested matched case-control study and the primary analyses were based on conditional logistic regression.
To exemplify these methods we utilized data from a nested case control study examining repeated measures of urinary phthalate metabolites during pregnancy in association with preterm birth.
We utilize the microlocal analysis of the wave operator □="∂t2−Δx and multiplication by a nested conormal distribution in order to study their action on spaces of conormal-like distributions.
The vif sequence was amplified by a nested PCR.
Amplification of the ITS was performed by a nested PCR.
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